Primary Research Paper
METHODS
Explain how the materials were prepared and why these methods are the best approach for answering your research question(s).
Explain how you analyzed your results.
Always written in passive voice. Passive voice puts the recipient of the action at the beginning of the sentence. Notice this sentence: “The soak water was drained from the soybeans which were then blanched…”.
Soymilk preparation: To prepare soymilk, 100 g of soybeans were soaked for 14–16 h in 1 L of distilled water at room temperature (28 ∘C) in a 2 L beaker. The soak water was drained from the soybeans, which were then blanched at 98 ∘C in boiling distilled water for 30 min. The beans were hand-washed thoroughly to remove their testa. The soybeans and 600 mL of boiled distilled water at 87–90 ∘C were placed in a blender (Bajaj, India) and blended for 3 min. The boiled water inactivated the lipoxygenase enzyme during blending. The resulting slurry was filtered through two layers of muslin cloth. Approximately 600 mL of soymilk was obtained per 100 g of soybeans
MATERIALS
Describe the materials used in the study, where/how they were obtained, and any other necessary storage or travel information.
Just like with methodology, materials are always written in passive voice.
Passive voice is when you move the object to the front of the sentence and the subject to the end. For example:
Active: The dog chased the rat.
Passive: The rat was chased by the dog.
Source of cultures: The bacterial strains used (L. rhamnosus NCDC19 and NCDC24) were obtained from the National Collection of Dairy Cultures (NCDC, Karnal, India) or from cheese isolates in our laboratory (Bioactive Peptides Laboratory, NDRI, Karnal, India). The cultures were maintained by biweekly transfers into sterile litmus milk or soymilk and held at 5 ∘C between transfers